Biochemical characterization of rice glutelin
WebNov 2, 2008 · Abstract. A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the … WebSep 1, 2024 · Here we investigated and characterized the formation of thermal-induced rice glutelin fibrils at 85 °C and pH 2.0. The potential effects of rice glutelin fibrils on the in vitro starch digestibility were also evaluated. Thioflavin T fluorescence spectral analysis revealed extensive formation of rice glutelin fibrils after 4 h of incubation at pH 2.0 and 85 °C.
Biochemical characterization of rice glutelin
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WebINTRODUCTION. The major endospermic rice protein is glutelin,[1, 2] which comprised up to 70 to 80% of total rice protein.Janick also found 70% of glutelin protein in rice flour as compared to other cereals, which were higher in prolamin contents. The biochemical characterization of rice proteins shows the important nutritional properties and these … WebThe rice (Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis analysis revealed that the mutant lacked the polypeptide pI6.71/α-2 encoded by glu4 while forming a new polypeptide of pI6.50/α-1. Experiments were conducted to identify the …
WebNov 2, 2008 · A new glutelin gene, designated GluD-1, has been discovered by comparing the seed storage proteins from 48 japonica and indica rice cultivars on SDS-PAGE gels. Evidence that GluD-1 is a member of the glutelin family was provided by Western blots using anti-glutelin antiserum and by mapping the gene to the chromosomal glutelin …
WebJun 1, 1985 · The two major subunits of rice glutelin, the acidic (alpha) and basic (beta) polypeptides were purified by chromatofocusing and cation … WebApr 1, 2002 · Very little is known about the gene that regulates the biochemical and cellular processes responsible for the formation of PBs. Rice mutants that show variations in the profile patterns of storage proteins have been identified and assigned into four classes (Kumamaru et al., 1988; Ogawa et al., 1989).
WebThe rice ( Oryza sativa L.) mutant of glu4a, lacking the glutelin α-2 subunit while the α-1 subunit increased (α-1H/α-2L), was used in this study. Two-dimensional electrophoresis …
WebJun 15, 2009 · In this study, transgenic a123 expressing glutelin with a mutation at the processing site proved to be useful for studying the glutelin sorting and assembly system in rice seeds. The results obtained using transgenic rice plants clearly address the functional role of post-translational processing from the glutelin precursor to the mature acidic ... dutch oven baked hamWebJun 1, 2003 · L. Q. Qu 81-298-38-8373 81-298-38-8397 [email protected] X. L. Wei H. Satoh T. Kumamaru M. Ogawa F. Takaiwa Institute of Genetics and Developmental Biology, the Chinese Academy of Sciences, Beijing 100101, China Institute of Genetic Resources, Faculty of Agriculture, Kyushu University, Hakozaki 6-10-1, Fukuoka 812-8581, Japan … in 2 memorabilia waurn pondsWebJan 1, 1992 · The 51-kDa protein is the rice glutelin precursor, which is processed to give rise to the 34-37-kDa u-subunit and 21-23-kDa subunit [3,7]. Similarly, the 14-kDa protein has been identified as a rice prolamine [7]. The 25- and 16-kDa proteins were the most abundant polypeptides in the globulin fraction (Fig. 1, lane C). ... Biochemical ... in 2 months timeWebDec 31, 2024 · Enhancing the solubility of rice glutelin in neutral aqueous solution is the prerequisite for the development of rice protein drinks and ingredients. Herein, glutelin … in 2 racingWebRice Glutelins SpringerLink Seed Proteins Cite as Rice Glutelins Fumio Takaiwa, Masahiro Ogawa & Thomas W. Okita Chapter Circuit is open Abstract Despite being the major … in 2 plumbingNational Center for Biotechnology Information dutch oven bavarian pot roast recipeWebRice glutelin cDNA clones isolated from a rice cDNA library constructed from immature endosperm encoded polypeptides containing the same number of amino acids including a 24 amino acid signal peptide, a 282 amino acid acidic subunit and a 193 amino acid basic subunit. SummaryRice glutelin cDNA clones were isolated from a rice cDNA library … dutch oven bbq chicken thighs