WebThe results suggested that MiMammal primers are capable of amplifying and distinguishing a diverse group of mammalian species. In addition, analyses of water samples from zoo cages of mammals with known species composition suggested that MiMammal primers could successfully detect mammalian species from water samples in the field. Then, we ...
哺乳類の網羅的検出系:MiMammalの開発｜はじめての環境DNA
WebMiMammalとMiBird、MiFishと増幅領域は同じだし、配列もよく似ている。 よく似ているということは、プライマーでは分けられないということ。 結構困りますね。 Web3種類のユニバーサルプライマーを用いて神奈川県中部に生息す ... the MiMammal primer set, and mitochondrial 16S rRNA region of the gBird primer set were amplified using the following primers: 5´-GGWACWGGWTGAACWGT WTAYCCYCC-3´ (IntF) and 5´-TAHACTTCNGGGTGKCCRAARAATCA-3´ dapagliflozin linagliptin metformin rat
Environmental DNA metabarcoding reveals the presence of a
WebPubMed WebJun 11, 2024 · The MiMammal-U primer set targets an ~ 200 bp region of the 12S locus and was created to specifically amplify mammal DNA from environmental samples 38. We … Web145 our primers MiMammal-U (Table 1). In addition, we also designed MiMammal-E and 146 MiMammal-B primers after preliminary experiments to accommodate sequence variations in 147 the priming sites of elephants and bears, respectively. Primer sequences with MiSeq adaptors 148 (for the first- and second-round PCR) are listed in Table 1. dapagliflozin pbs