Phm1 vector

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Webb12 aug. 2024 · Plasmid pHM1-XocRNA-CSpec1-CSpec2 targeting the spectinomycin resistant gene was transferred into PXO99 A cells and plated on spectinomycin-containing medium. Only 8 spectinomycin resistant transformants were obtained, but lots of colonies were observed to form when using the control vector ( Figure 1C ). WebbAbstract: The promoter region of Ralstonia solanacearum RipAK gene was PCR amplified, and fused with lacZ reporter gene to generate pHM1:P RiPAK LacZ. β-galactosidase …

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WebbWe developed a modular plasmid assembly system to transfer DNA modules from entry vectors into high‐copy number pHM1‐derived vectors and use it for protein detection, … Webb3 apr. 2024 · rpfC containing a point mutation was provided in trans in the ΔrpfFΔrpfC double mutant on a pHM1 vector. The P engXcc-GUS sequence was fused in the … cisf hc min admit card download 2015

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WebbAbstract: The promoter region of Ralstonia solanacearum RipAK gene was PCR amplified，and fused with lacZ reporter gene to generate pHM1：PRiPAKLacZ. β … WebbPHM1-41 are fibroblasts isolated from the uterus of a pregnant female. They are useful for long-term studies as their behavior is quite reproducible. Log In ... infected at passage 2 … WebbGenetics. 4. Genetics. 4.1. Genetic Tools. A variety of genetic tools are available for methanotrophs and have been successfully applied for decades. Replicating vectors are … cisf forms

An improved, versatile and efficient modular plasmid assembly …

Molecular Plant Pathology

Webb31 okt. 2024 · 0.5 kb, 20.45% ratio) and large (> 1 kb, 54.55% ratio) DNA fragment deletions in PXO99A after DSB repair via both NHEJ and AEJ pathways (S1E and S1F Fig http://www.methanotroph.org/wiki/genetics/ cisf.gov.in apply onlineWebbTaxonomy - vectors (no rank) Download. View proteins. Mnemonic name. 9ZZZZ. Taxon ID. 29278. Scientific name. vectors. Parent. artificial sequences. Children. Sequencing vector pAA3.7X; Recombinant plasmid pMM25-3; Recombinant plasmid pMM5-3; Recombinant plasmid pMM5-5; Cloning vector M13mp18; Browse all direct children (14,145) diamond supply co clothing online

"WebbpHM1, M0163 stain containing a blank pHM1 vector, Rif R, Sp R ΔctrA-ctrA, genetic complementary strain of ctrA. M0163 strain containing a recombinant pHM1:: ctrA … " - Phm1 vector

Phm1 vector

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Webb26 dec. 2024 · Therefore, we constructed a helper λ‐Red recombination system into the pHM1 vector, yielding pHM1‐λRed (Figure 3b), and transformed this into Xoo to improve … Webb19 juli 2016 · Empty pHM1 vector was also introduced into mutant strains. Spectinomycin-resistant putative complementation clones were confirmed by PCR and sequencing and …

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Webb1 apr. 2007 · The broad host-range vector pHM1 (Yang et al., 2000) was used in complementation experiments. Concentrations of the antibiotics used were as follows: … Webb6 maj 2024 · The 445-bp promoter region of the RSc0454 gene was fused with the LacZ gene in the pLacZ-Basic vector (Clontech) at KpnI-XhoI sites. Next, the promoter region, …

Webb15 maj 2024 · Taq polymerase from KAPA Biosystems (Wilmington, Massachusetts, USA) was used for all screening purposes. For cloning in the pHM1 vector, the primers as … Webb20 okt. 2004 · The plasmid obtained, designated pBSPIPORF1, was digested with EcoRI and HindIII and cloned into the broad-host-range vector pHM1 to generate …

Webb5 juli 2024 · 110 introduction of the xopQ wildtype gene into the xopQ-strain through the pHM1 vector. 111 Notably, the xopQ D116A and xopQ Y279A mutants failed to … WebbpHom-1. Mammalian vector encoding an FKBP homodimerizer domain, for creating soluble fusion proteins that can be dimerized with a drug. Also known as pC4-FV1E. Sequence …

WebbHover: Reveals additional information for enzyme cut sites, ORFs, feature descriptions, and primer details. Select: Click one location on the plasmid and then click on a second …

Webb17 jan. 2024 · To modify the Agrobacterium CBE, the destination vector was replaced with the Xanthomonas-compatible vector pHM1, a broad host range plasmid derived from … diamond supply co clothes cheapWebb属于主要同源群，GC含量为35%，包含一个主要的开放阅读框945bp（orfa）。所预测的蛋白质，命名为Rep A，与一组通过ssDNA中间产物（ssDNA质粒）复制的质粒中的复制蛋白具有序列同源性。pST1的限制性内切酶分析显示与嗜热链球菌质粒pHM1和pER8相似。宿 … cisf hchttp://biovector.net/product/319420.html diamond supply co coach jacketWebb24 jan. 2024 · Because of the genetically stable replication and delivery efficiency, pHM1 is the most widely used cloning vector in X. oryzae , especially in the model strain Xoo … cisf hc admit card 2019WebbCosmid vector pHM1 Taxonomy ID: 411666 (for references in articles please use NCBI:txid411666) current name. Cosmid vector pHM1. NCBI BLAST name: other … diamond supply co fashionWebbExcited to share the Scale and Accenture partnership! Together, we're going to be bringing AI to Enterprises and Government. diamond supply co coupon codesWebbpHM was a gift from Markus Ralser (Addgene plasmid # 64173 ; http://n2t.net/addgene:64173 ; RRID:Addgene_64173) For your References section: … cisf hcm pst