Sirt3 pcr
WebAug 2, 2024 · A SIRT3 mRNA expression was measured by qRT-PCR in MCF7 cells. B SIRT3 mRNA expression was measured by qRT-PCR in MDA-MB-231 cells. C SIRT3 protein expression was measured by Western blotting in MCF7 and MDA-MB-231 cells. D Quantitative analysis of SIRT3 protein expression was shown. WebThe mitochondrial SIRT3 protein may be implicated in these processes as it is able to sense the metabolic state of the cell and alter mitochondrial protein function post-translationally. …
Sirt3 pcr
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WebJun 1, 2024 · Decreased expression of mitochondrial Sirt3 (28kD) was confirmed in Sirt3 knockdown cells by real-time PCR as well as immunoblotting (Fig. 1 A and … WebIn humans, cardiac SIRT3 expression was reduced in aged females, but not in males, and this associated with a pro-inflammatory phenotype, as indicated by the increased number …
WebSep 23, 2008 · Sirt3 −/− MEFs were transiently transfected with an empty vector, wild-type Sirt3, or a deacetylase-inactive form of Sirt3, Sirt3 (HY). All transfections were performed with a cotransfected GFP expression vector to allow for subsequent purification of transfected cells using FACS sorting. WebJul 22, 2010 · Sirt3 is a member of the class III histone deacetylases, also referred to as sirtuins (SIRTs). The SIRTs are a conserved family of proteins possessing NAD + …
WebApr 12, 2024 · Zhou et al. find that IL-4 activates SENP1-Sirt3 signaling in mitochondria to deacetylate glutamate dehydrogenase 1 (GLUD1) to promote glutaminolysis and α-ketoglutarate ... Real-time PCR analysis on Arg1, Retnla, or Chil3 expression in Sirt3 WT or Sirt3 KR BMDMs treated with vehicle (Ctr), IL-4, or IL-4 plus Etomoxir (3 μM) for 24 h; IL … WebSIRT3 regulates glucose and lipid metabolism and maintains myocardial ATP levels, which protects the heart from metabolic disturbances. SIRT3 can also protect cardiomyocytes …
WebAug 3, 2009 · We demonstrate that SIRT3 is a stress-responsive deacetylase that blocks the cardiac hypertrophic response through activation of Foxo-dependent antioxidants, …
Web万方 知网 维普网 万方医学 《细胞与分子免疫学杂志》官网 查看更多 钛学术 (全网免费下载) journal.9med.net 钛学术 code on ge dryerWebApr 10, 2024 · SIRT3 encodes a member of the sirtuin family of class III histone deacetylases, homologs to the yeast Sir2 protein. The encoded protein is found exclusively in mitochondria, where it can eliminate reactive oxygen species, inhibit apoptosis, and prevent the formation of cancer cells. code online phpWeb【摘 要】本试验旨在原核表达猪去乙酰化酶SIRT3,并初步鉴定重组蛋白的抗原性和特异性.采用RT-PCR扩增包含SIRT3的完整编码序列;通过Ω-PCR,将SIRT3完整编码序列克隆至原核表达载体pET-28a(+)中,转入大肠杆菌Rosetta(DE3)中进行诱导表达和亲和层析纯化;经免疫印迹 … code online angularWebFeb 1, 2024 · Identification of ARE DNA-binding sites in the promoter region of SIRT3 gene. (A) qRT-PCR assay for SIRT3 in HEK293 cells with NRF2 overexpression for 24 h (n = 5 … calories in spicy mayoWebFeb 28, 2024 · SIRT3-Dependent Mitochondrial Dynamics Remodeling Contributes to Oxidative Stress-Induced Melanocyte Degeneration in Vitiligo Mitochondrial dysregulation has been implicated in oxidative stress-induced melanocyte destruction in vitiligo. However, the molecular mechanism underlying this process is merely investigated. code online nmcWebAug 30, 2024 · SIRT3, a member of the silent information regulator (Sirtuins) family, participates in cell biological functions such as energy metabolism and cell aging by regulating mitochondrial function, and it is closely related to tumor development and progression [4–6]. code online officeWebJan 11, 2013 · H9c2 cells were treated with H 2 O 2 (50 μM, 6 h) and SIRT3 mRNA and protein levels were determined by RT-PCR and western blot analysis, respectively. H9c2 cells treated with H 2 O 2 exhibited significantly decreased SIRT3 mRNA and protein expression (Fig. 2).Moreover, pretreatment with resveratrol significantly increased SIRT3 … code online website