Small highly active dnas that hydrolyze dna
Webb5 juni 2013 · Natural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn(2+), … Webb(B) Hydrolytic instability of natural DNA sequences. Trace amounts of each natural DNA (5′ 32P-labeled) were incubated at 37 °C in reaction buffer without or with 2 mM Zn2+ for 6 …
Small highly active dnas that hydrolyze dna
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WebbNatural DNA sequences conforming to the class I consensus sequence and structure were found that undergo hydrolysis under selection conditions (2 mM Zn 2+, pH 7), which … WebbSmall, Highly Active DNAs That Hydrolyze DNA: 23679108: 10.1021/ja403585e: 2013: Liu, Y. He, J. Breaking the conservation of guanine residues in the catalytic loop of 10–23 DNAzyme by position-specific nucleobase modifications for rate enhancement: 23615852: 10.1039/c3cc42067a: 2024: Lee, Y. Silverman, S. K
Webb6 juni 2013 · DNA phosphoester bonds are exceedingly resistant to hydrolysis in the absence of chemical or enzymatic catalysts. This property is particularly important for … WebbThe development of a simple DNA sensor based on DNAzyme and PCR and aimed at the detection of low concentrations of zinc (II) ions, which showed sensitivity (10 nM) and high specificity for zinc ion detection is described. AbstractThe development of a simple sensor (9NL27-Zn) based on DNAzyme and PCR and aimed at the detection of low …
WebbSmall, highly active DNAs that hydrolyze DNA. Article. Full-text available. May 2013; J AM CHEM SOC; Hongzhou Gu; Kazuhiro Furukawa; Zasha Weinberg; Daniel F Berenson; Ronald R Breaker; Webbhydrolyzing DNAs is further highlighted by the fact that it has been relatively straightforward to create deoxyribozymes that promote DNA cleavage by oxidative 4 or …
WebbSmall, highly active DNAs that hydrolyze DNA. J. Am. Chem. Soc. 2013; 135: 9121-9129. Crossref; PubMed; Scopus (94) Google Scholar; of I-R3 in DNA cleavage. Besides I-R3, other metal-dependent deoxyribozymes that cleave a RNA bond have also been successfully engineered as molecular triggers to actively generate nanoholes in DNA origami ...
Webb6 jan. 2016 · Nature - Both DNA and RNA molecules are foldable and can adopt conformations that exhibit catalytic activity. While the structures of various catalytic RNAs — or ribozymes — have been... green farm washington njWebbCircLigase is a thermostable ATP-dependent single-stranded DNA ligase that catalyzes intramolecular ligation of single-stranded DNA templates. The ligation substrates must … fluke direct ordersWebb6 juni 2013 · The identification of small DNA-cleaving deoxyribozymes that function with high speed under mild reaction conditions should permit their use in various … fluke distributors in south africaWebbLigation of linear single-stranded DNAs with CircLigase to form circles. CircLigase is a thermostable ATP-dependent single-stranded DNA ligase that catalyzes intramolecular ligation of single-stranded DNA templates. The ligation substrates must carry 5′-phosphate a 3′-hydroxyl groups on the donor and acceptor DNAs, respectively. fluke dms professionalWebb14 okt. 2024 · Catalytic DNAs, mainly DNA/RNA-cleaving DNAs, ... with the total 17 conserved nucleotides forming a catalytic loop to site-specifically hydrolyze DNA in the loop upon the supplementation of Zn 2+ ... Small, highly active DNAs that hydrolyze DNA. J. Am. Chem. Soc., 135 (2013), pp. 9121-9129. fluke dtx 1200 software downloadWebb1 juni 2013 · I-R3 DNAzyme is a small, highly active catalytic DNA for DNA hydrolysis. In here, we designed two cis-structure DNAzymes (I-R3N and I-R3S) based on the different locates of the joint linker ... green fascinator hats for womenWebbDNA cleavage by 10MD5 proceeds with kobs = 2.7 h −1 and rate enhancement of 10 12 over the uncatalyzed P−O hydrolysis reaction. 10MD5 has a very sharp pH optimum near 7.5, with greatly reduced DNA cleavage rate and yield when the pH is changed by only 0.1 unit in either direction. fluke disease fish